Figure 2.

Silver and methylene-blue staining of Wolbachia endobacteria. (A, C) Cross sections of female O. volvulus show Wolbachia (arrow-heads) in the lateral cords of the hypodermis. (E) Longitudinal section of O. jakutensis with black endobacteria in hypodermis and oocytes (arrowheads). (B, D, F) No endobacteria are found in the lateral cords or in oocytes of female L. loa worms. lc, lateral cord; o, oocytes in the ovary; u, uterus. A – D, semi-thin sections stained with azure II and methylene-blue; E, F, sections stained with silver, Warthin-Starry method; scale bar = 25 μm.