Figure 6.

Wolbachia in microfilariae of Onchocerca but not of L. loa and the reaction of neutrophils. (A – D) Antiserum against Wol-Di-WSP stains the Wolbachia (arrowheads) of microfilariae in the uterus of O. volvulus (A) and in an onchocercoma (C) whereas no bacteria are seen in intact microfilariae of L. loa in the uterus (B) or in human spleen tissue (D). (E – H) Neutrophil granulocytes are attracted to degenerating microfilariae (arrows) of O. volvulus in lymph node tissue 24 hours after a dose of ivermectin (E, anti-neutrophils elastase) or in an onchocercoma of an untreated patient (G, anti-neutrophils defensins). Neutrophils are not attracted by degenerated L. loa microfilariae (arrows) in spleen tissue from a human patient not treated with microfilaricidal drugs (F, anti-neutrophils elastase) or from an experimentally infected monkey (H, anti-neutrophils defensins). n, neutrophil granulocytes; scale bar = 25 μm.