Figure 3.

(A) Single worm PCR of transgenic worms, 49cAv17 and 49gAv17, containing the cDNA (lane 1) and genomic sequence (lane 2) of A. viteae cystatin respectively. (B) RT-PCR analysis of transcripts from transgenic worms, 49cAv17 and 49gAv17, containing the cDNA (lane 2) and genomic sequence (lane 3) of A. viteae cystatin respectively. Negative control with wild type C. elegans in lane 4 and marker in lane 1. (C) Western Blot with anti-His antibodies. Lane 1: Transgenic C. elegans expressing Av17 constitutively (103cAv173') ; Lane 2: Transgenic C. elegans (49cAv17) expressing Av17 induced by heat shock ; Lane 3: pha-1 control worms