|
Results for Wuchereria bancrofti DNA extraction in pools of laboratory-reared uninfected mosquitoes added (positive) or not (negative) with one experimentally infected mosquito. |
|||||
| Pool size |
Method A (Reference) |
DNA sample fraction |
191 bp band |
225 bp band |
Résults * |
|
|
|||||
| 1 |
positive controls |
1/20e |
+ (n = 3/3) |
+ (n = 3/3) |
+ (n = 3/3) |
| negative control |
1/20e |
- (n = 1) |
+ (n = 1) |
- (n = 1) |
|
| 20 |
positive controls |
1/20e |
+ (n = 2/3) |
+ (n = 3/3) |
+ (n = 2/3) |
| negative control |
1/20e |
- (n = 1) |
+ (n = 1) |
- (n = 1) |
|
| 30 |
positive controls |
1/20e |
+ (n = 3/3) |
+ (n = 3/3) |
+ (n = 3/3) |
| negative control |
1/20e |
- (n = 1) |
+ (n = 1) |
- (n = 1) |
|
| 50 |
positive controls |
1/20e |
+ (n = 3/3) |
+ (n = 3/3) |
+ (n = 3/3) |
| negative control |
1/20e |
- (n = 1) |
+ (n = 1) |
- (n = 1) |
|
|
|
|||||
| Pool size |
Method B (Qiagen kit) |
DNA sample fraction |
191 bp band |
225 bp band |
Résults |
|
|
|||||
| 1 |
positive controls |
1/20e |
+ (n = 3/3) |
+ (n = 3/3) |
+ (n = 3/3) |
| 1/40e |
+ (n = 3/3) |
+ (n = 3/3) |
+ (n = 3/3) |
||
| 1/240e |
+ (n = 3/3) |
+ (n = 3/3) |
+ (n = 3/3) |
||
| negative control |
1/20e |
- (n = 1) |
+ (n = 1) |
- (n = 1) |
|
| 1/40e |
- (n = 1) |
+ (n = 1) |
- (n = 1) |
||
| 1/240e |
- (n = 1) |
+ (n = 1) |
- (n = 1) |
||
| 20 |
positive controls |
1/20e |
- (n = 3/3) |
- (n = 3/3) |
inh (n = 3/3) |
| 1/40e |
- (n = 3/3) |
- (n = 3/3) |
inh (n = 3/3) |
||
| 1/240e |
+ (n = 2/3) |
+ (n = 2/3) |
inh (n = 1/3) |
||
| 1/1200e |
+ (n = 3/3) |
+ (n = 3/3) |
+ (n = 3/3) |
||
| negative control |
1/20e |
- (n = 1) |
- (n = 1) |
inh (n = 1) |
|
| 1/40e |
- (n = 1) |
- (n = 1) |
inh (n = 1) |
||
| 1/240e |
- (n = 1) |
- (n = 1) |
inh (n = 1) |
||
| 1/1200e |
- (n = 1) |
+ (n = 1) |
- (n = 1) |
||
| 30 |
positive controls |
1/20e |
- (n = 3/3) |
- (n = 3/3) |
inh (n = 3/3) |
| 1/40e |
- (n = 3/3) |
- (n = 3/3) |
inh (n = 3/3) |
||
| 1/240e |
+ (n = 2/3) |
+ (n = 3/3) |
+ (n = 2/3) |
||
| negative control |
1/20e |
- (n = 1) |
- (n = 1) |
inh (n = 1) |
|
| 1/40e |
- (n = 1) |
- (n = 1) |
inh (n = 1) |
||
| 1/240e |
- (n = 1) |
+ (n = 1) |
- (n = 1) |
||
| 50 |
positive controls |
1/20e |
- (n = 3/3) |
- (n = 3/3) |
inh (n = 3/3) |
| 1/40e |
- (n = 3/3) |
- (n = 3/3) |
inh (n = 3/3) |
||
| 1/240e |
+ (n = 3/3) |
+ (n = 3/3) |
+ (n = 3/3) |
||
| negative control |
1/20e |
- (n = 1) |
- (n = 1) |
inh (n = 1) |
|
| 1/40e |
- (n = 1) |
- (n = 1) |
inh (n = 1) |
||
| 1/240e |
- (n = 1) |
+ (n = 1) |
- (n = 1) |
||
Plichart et al. Filaria Journal 2006 5:2 doi:10.1186/1475-2883-5-2 |
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