|
PCR amplification of Wuchereria bancrofti DNA in pools of laboratory-reared uninfected mosquitoes spiked with microfilariae |
||||
| Pool size |
Control testing |
191 bp band |
225 bp band |
Résults |
|
|
||||
| 0 |
Mf spiked controls |
+ (n = 3/3) |
+ (n = 3/3) |
+ (n = 3/3) |
| Negative control |
- (n = 1) |
+ (n = 1) |
- (n = 1) |
|
| 20 |
Mf spiked controls |
+ (n = 3/3) |
+ (n = 3/3) |
+ (n = 3/3) |
| Negative control |
- (n = 1) |
+ (n = 1) |
- (n = 1) |
|
| 30 |
Mf spiked controls |
+ (n = 3/3) |
+ (n = 3/3) |
+ (n = 3/3) |
| Negative control |
- (n = 1) |
+ (n = 1) |
- (n = 1) |
|
| 50 |
Mf spiked controls |
+ (n = 3/3) |
+ (n = 3/3) |
+ (n = 3/3) |
| Negative control |
- (n = 1) |
+ (n = 1) |
- (n = 1) |
|
|
* DNA extracted according the B method; sample fraction used 1/240e | ||||
Plichart et al. Filaria Journal 2006 5:2 doi:10.1186/1475-2883-5-2 |
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