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Summary of long-term trial 1: parasite mean motility scores and viability (MTT colorimetry) |
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| Compound/drug and conc. |
Mean motility scores over a period of 40 days |
Motility |
MTT |
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|
|
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| DAY |
1 |
5 |
10 |
15 |
20 |
25 |
30 |
35 |
40 |
% reduction on day 40 (P) |
% inhibition on day 40 (P) |
| Control |
8.0 |
7.4 |
7.1 |
7.0 |
5.3 |
5.5 |
6.3 |
6.4 |
4.9 |
||
| CGP 6140 1.25 × 10-5M (positive control) |
0.5 |
0.8 |
0.0 |
0.5 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
100.0 (0.005) |
83.3 (0.098) |
| Rifampicin 5 × 10-5M |
7.7 |
6.8 |
6.7 |
6.8 |
3.2 |
1.7 |
0.2 |
0.2 |
0.0 |
100.0 (0.002) |
89.6 (0.033) |
| Rifampicin 1.25 × 10-5M |
7.0 |
7.0 |
7.3 |
5.7 |
6.5 |
5.2 |
5.7 |
5.0 |
5.6 |
0.0 (0.647) |
0.0 (0.798) |
| Minocycline 5 × 10-5M |
8.0 |
6.7 |
7.3 |
5.7 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
100.0 (0.002) |
84.1 (0.042) |
| Minocycline 1.25 × 10-5M |
7.8 |
7.5 |
6.7 |
5.7 |
4.3 |
2.5 |
4.8 |
1.7 |
0.0 |
100.0 (0.002) |
93.7 (0.026) |
| Doxycycline 5 × 10-5M |
8.3 |
8.0 |
8.0 |
5.7 |
0.3 |
0.0 |
0.0 |
0.0 |
0.0 |
100.0 (0.002) |
93.0 (0.027) |
| Doxycycline 1.25 × 10-5M |
7.3 |
6.5 |
6.3 |
4.8 |
6.3 |
6.3 |
6.2 |
6.2 |
5.5 |
0.0 (0.699) |
0.0 (0.801) |
| Ethambutol 5 × 10-5M |
6.8 |
6.8 |
6.5 |
6.7 |
4.0 |
4.8 |
4.3 |
5.0 |
2.7 |
44.9 (0.170) |
73.0 (0.108) |
| Dapsone 5 × 10-5M |
7.3 |
7.3 |
7.7 |
5.0 |
6.8 |
7.0 |
7.2 |
7.3 |
6.5 |
0.0 (0.250) |
0.0 (0.798) |
| Pyrazinamide 5 × 10-5M |
7.7 |
7.7 |
7.2 |
3.5 |
5.5 |
5.5 |
5.7 |
5.2 |
3.7 |
24.5 (0.503) |
4.8 (0.949) |
|
The effects of exposure to a range of antibiotics on the viability of Onchocerca gutturosa adult males in long-term (40 day) in vitro culture. Viability was assessed by measuring worm motility scores throughout the trial and by MTT colorimetry on day 40. | |||||||||||
Townson et al. Filaria Journal 2006 5:4 doi:10.1186/1475-2883-5-4 |
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