|
Summary of long-term trial 2: parasite mean motility scores and viability (MTT colorimetry) |
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| Compound/drug and conc. |
Mean motility scores over a period of 40 days |
Motility |
MTT |
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|
|
|||||||||||
| DAY |
1 |
5 |
10 |
15 |
20 |
25 |
30 |
35 |
40 |
% reduction on day 40 (P) |
% inhibition on day 40 (P) |
|
|
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| Control |
7.6 |
7.2 |
7.1 |
6.8 |
5.9 |
4.9 |
5.8 |
6.1 |
6.3 |
||
| aCGP 6140 1.25 × 10-5M (positive control) |
1.3 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
100.0 (ND) |
87.2 (ND) |
| bNorfloxacin 5 × 10-5M |
6.9 |
7.4 |
7.1 |
2.4 |
2.5 |
2.6 |
1.4 |
1.0 |
2.5 |
60.3 (ND) |
90.7 (ND) |
| Ciprofloxacin 5 × 10-5M |
6.9 |
7.9 |
7.1 |
3.6 |
4.1 |
3.0 |
2.3 |
2.5 |
3.0 |
52.4 (0.080) |
73.3 (0.040) |
| Vancomycin 5 × 10-5M |
6.4 |
7.4 |
6.5 |
3.6 |
3.6 |
2.8 |
2.4 |
1.6 |
2.1 |
66.7 (0.009) |
83.7 (0.022) |
| Gentamycin 5 × 10-5M |
7.6 |
5.5 |
6.3 |
2.8 |
2.6 |
0.8 |
1.5 |
1.0 |
1.6 |
74.6 (0.005) |
83.7 (0.017) |
| Ceftriaxone 5 × 10-5M |
7.1 |
6.8 |
7.0 |
2.4 |
2.0 |
0.9 |
0.6 |
0.6 |
0.7 |
88.9 (<0.001) |
90.7 (0.011) |
| cTriclosan 5 × 10-5M |
6.9 |
4.9 |
0.4 |
0.1 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
100.0 (ND) |
98.8 (ND) |
| cCerulenin 5 × 10-5M |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
100.0 (ND) |
100.0 (ND) |
|
a. Day 40 results based on 1 worm; 5 worms lost to microbial contamination b. Day 40 results based on 2 worms; 4 worms lost to microbial contamination c. Denotes compounds that were toxic to the monkey kidney cell feeder layer The effects of exposure to a range of antibiotics on the viability of Onchocerca gutturosa adult males in long-term (40 day) in vitro culture. Viability was assessed by measuring worm motility scores throughout the trial and by MTT colorimetry on day 40. | |||||||||||
Townson et al. Filaria Journal 2006 5:4 doi:10.1186/1475-2883-5-4 |
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