Table 3

Summary of long-term trial 3: parasite mean motility scores and viability (MTT colorimetry)

Compound/drug and conc.
 Mean motility scores over a period of 40 days
 Motility
 MTT
DAY
 1
 5
 10
 15
 20
 25
 30
 35
 40
 % reduction on day 40 (P)
 % inhibition on day 40 (P)
Control
 8.4
 8.3
 7.6
 7.5
 7.8
 7.7
 7.6
 7.2
 7.2

CGP 6140 1.25 × 10-5M (positive control)
 2.5
 0.3
 0.0
 0.0
 0.0
 0.0
 0.0
 0.0
 0.0
 100.0 (<0.001)
 67.4 (0.0032)
Rifampicin 5 × 10-5M
 8.0
 6.6
 2.1
 0.4
 0.4
 0.0
 0.0
 0.0
 0.0
 100.0 (<0.001)
 67.4 (<0.001)
Erythromycin 5 × 10-5M
 8.4
 8.5
 8.0
 7.6
 7.7
 7.6
 7.4
 7.5
 7.4
 0.0 (0.539)
 0.0 (0.210)
Oxytetracycline 5 × 10-5M
 8.0
 8.1
 7.7
 7.5
 7.6
 7.5
 7.1
 6.4
 6.9
 4.2 (0.360)
 0.0 (0.995)
Isoniazid 5 × 10-5M
 8.2
 8.2
 7.7
 8.0
 7.7
 7.3
 6.8
 5.7
 5.2
 27.8 (0.010)
 35.5 (0.074)
Isoniazid 5 × 10-5M + Rifampicin 5 × 10-5M
 7.8
 8.2
 5.0
 4.0
 2.7
 0.8
 0.0
 0.0
 0.0
 100.0 (<0.001)
 71.7 (0.002)
Trimethoprim 5 × 10-5M
 8.4
 8.4
 7.9
 7.6
 7.6
 8.0
 7.5
 7.0
 7.0
 2.8 (0.549)
 0.0 (0.319)
Sulphamethoxazole 5 × 10-5M
 8.1
 8.3
 8.4
 7.6
 7.6
 8.0
 7.5
 7.3
 7.1
 1.4 (0.848)
 0.0 (0.998)
Trimeth. 5 × 10-5M + Sulphamethox. 5 × 10-5M
 8.5
 8.4
 8.0
 7.5
 6.8
 7.5
 7.1
 7.4
 6.9
 4.2 (0.449)
 0.0 (0.828)
Clofazimine 1.25 × 10-5M
 7.7
 6.5
 5.2
 3.5
 4.3
 3.5
 3.2
 0.8
 0.0
 100.0 (<0.001)
 77.4 (<0.001)
Clofazimine 3.13 × 10-6M
 8.2
 7.0
 4.5
 5.5
 5.0
 4.8
 3.4
 1.2
 0.8
 88.9 (<0.001)
 53.3 (0.028)

The effects of exposure to a range of antibiotics and antibiotic combinations on the viability of Onchocerca gutturosa adult males in long-term (40 day) in vitro culture. Viability was assessed by measuring worm motility scores throughout the trial and by MTT colorimetry on day 40.

Townson et al. Filaria Journal 2006 5:4   doi:10.1186/1475-2883-5-4